Research Article
Metabarcoding and Metagenomics 7: e102455
https://doi.org/10.3897/mbmg.7.102455 (25 Jul 2023)
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Chironomid origin
    • Size-sorting and tissue preparation
    • Sanger-sequencing and identification of individual specimens
    • DNA extraction
    • Artificial community composition
      • Experiment 1: Effect of size-sorting and tissue preparation on read abundance of specimens in artificial communities
      • Experiment 2: Effect of DNA input variation on read abundance of specimens in artificial communities
      • Experiment 3: Effect of DNA input dilutions on detection rates of specimens in artificial communities
      • Experiment 4: Natural communities under environmental stress
    • Metabarcoding
    • Bioinformatic analysis
    • Data preparation
      • Mock B
      • Natural communities
    • Statistical analysis
  • Results
    • Experiment 1: Effect of size-sorting and tissue preparation on read abundance of specimens in artificial communities
    • Experiment 2: Effect of DNA input variation on read abundance of specimens in artificial communities
    • Experiment 3: Effect of DNA input dilutions on detection rates of specimens in artificial communities
    • Experiment 4: Comparing standard versus dPCR metabarcoding approach for natural communities
  • Discussion
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary materials
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