Research Article
Metabarcoding and Metagenomics 7: e102455
https://doi.org/10.3897/mbmg.7.102455 (25 Jul 2023)
https://doi.org/10.3897/mbmg.7.102455 (25 Jul 2023)
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Article title
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Abstract
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Key words
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Introduction
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Materials and methods
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Chironomid origin
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Size-sorting and tissue preparation
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Sanger-sequencing and identification of individual specimens
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DNA extraction
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Artificial community composition
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Experiment 1: Effect of size-sorting and tissue preparation on read abundance of specimens in artificial communities
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Experiment 2: Effect of DNA input variation on read abundance of specimens in artificial communities
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Experiment 3: Effect of DNA input dilutions on detection rates of specimens in artificial communities
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Experiment 4: Natural communities under environmental stress
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Metabarcoding
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Bioinformatic analysis
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Data preparation
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Mock B
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Natural communities
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Statistical analysis
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Results
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Experiment 1: Effect of size-sorting and tissue preparation on read abundance of specimens in artificial communities
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Experiment 2: Effect of DNA input variation on read abundance of specimens in artificial communities
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Experiment 3: Effect of DNA input dilutions on detection rates of specimens in artificial communities
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Experiment 4: Comparing standard versus dPCR metabarcoding approach for natural communities
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Discussion
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Acknowledgements
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Additional information
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Conflict of interest
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Ethical statement
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Funding
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Author contributions
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Author ORCIDs
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Data availability
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References
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Supplementary materials
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