Linear regression models showing the direct relationship between relative proportion of HTS read abundance ~ relative proportion of species biomass for (A) COI and (B) 16S. The model fit was improved in each case by including terms relating to primer-template mismatches (Table 2). Insert boxplots showing the number of template-primer mismatches for each species in the COI and 16S dataset, respectively.

 
 
  Part of: Liu M, Burridge CP, Clarke LJ, Baker SC, Jordan GJ (2023) Does phylogeny explain bias in quantitative DNA metabarcoding? Metabarcoding and Metagenomics 7: e101266. https://doi.org/10.3897/mbmg.7.101266